Dr. Ranjan Dash

Dr. Ranjan DashMarquette University

Medical College of Wisconsin

MilwaukeeWI53201United States of America(414) 955-4497rdash@mcw.eduGoogle Scholar

Curriculum Vitae

Co-Director of Graduate Studies

Biomedical Engineering

Professor

MU-MCW Department of Biomedical Engineering

MCW Department of Physiology

Director

Computational Systems Biology Laboratory 

Professional Preparation

• Postdoc, 2006, Computational Systems Biology, Case Western Reserve University, Cleveland
• Postdoc, 2003, Computational Systems Biology, University of Washington, Seattle
• Postdoc, 2000, Computational Biofluid Dynamics, Texas A&M University, College Station
• Ph.D., 1998, Computational Biofluid Dynamics, Indian Institute of Technology, Delhi, India
• M.Sc., 1991, Applied Mathematics, Utkal University, Bhubaneswar, Orissa, India

Research Interests

• Computational Systems Biology & Bioengineering
• Mitochondrial, Cellular & Cardiovascular Physiology
• Cellular/Mitochondrial Metabolism & Redox Biology

Specific Research Interests

• Pulmonary and systemic microcirculatory gas exchange and acid-base regulation

• Physiologically-based pharmacokinetic modeling of drug transport and metabolism

• Integrated blood-tissue solute transport and cellular/mitochondrial metabolism

• Modeling and analysis of large-scale biochemical networks, protein-protein interaction networks, signaling network, and gene regulatory networks

• Cellular/mitochondrial calcium and reactive oxygen species homeostasis and signaling

• Cardiac electrophysiology, calcium handling, and excitation-contraction coupling

• Cardiac substrate and energy metabolism, oxidative stress, and ischemia-reperfusion injury

• Renal substrate and energy metabolism, oxidative stress, and salt-sensitive hypertension

Selected Recent Publications

Sadri S, Tomar N, Yang C, Audi SH, Cowley AW Jr, and Dash RK. Mechanistic computational modeling of the kinetics and regulation of NADPH oxidase 2 assembly and activation facilitating superoxide production. Free Rad Res 1-27, 2020.

Terhune SS^#,$, Jung Y^$, Cataldo K, and Dash RK^#. Network mechanisms and dysfunction within an integrated computational model of progression through mitosis in the human cell cycle. PLoS Comp Biol 16(4): e1007733, 2020. [# Equal contributions for senior authorship; ^$Equal contributions for first authorship]

Audi SH, Cammarata A, Dash RK, Clough AV, and Jacobs ER. Quantification of mitochondrial membrane potential in the isolated rat lung using rhodamine 6G. J Appl Physiol 128(4):892-906, 2020.

Korman B, Dash RK, and Peyton PJ. Elucidating the roles of solubility and ventilation-perfusion mismatch in the second gas effects using a two-step model of gas exchange. J Appl Physiol

Xie D, Audi SH, and Dash RK. A size modified Poisson-Boltzmann ion channel model in a solvent of multiple ionic species: Application to VDAC. J Comp Chem 41(3):218-230, 2020.

Haspula D, Vallejos A, Moore T, Tomar N, Dash RK, and Hoffmann BR. Influence of a hyperglycemic microenvironment on a diabetic versus healthy rat vascular endothelium reveals distinguishable mechanistic and phenotypic responses. Front Physiol 10:558, 2019.

Zhang X, Dash RK^#, Clough AV, Xie D, Jacobs ER, and Audi SH^#. Integrated computational model of lung tissue bioenergetics. Front Physiol 10:191, 2019. [# Equal contributions for senior authorship.]

Tomar N, Sadri S, Cowley AW Jr, Yang C, Qureshi N, Pannala VR, Audi SH, and Dash RK. A thermodynamically-constrained mathematical model for the kinetics and regulation of NADPH oxidase 2 complex-mediated electron transfer and superoxide production. Free Rad Biol Med 134:581-597, 2019.

Nasci VL, Chuppa S, Griswold L, Goodreau K, Dash RK, and Kriegel AJ. miR-21-5p regulates mitochondrial respiration and lipid content in H9C2 cells. Am J Physiol Heart Circ Physiol 316(3): H710-H721, 2019.

Korman B, Dash RK, and Peyton PJ. The effect of net gas volume changes on alveolar gas and arterial blood in the presence of ventilation-perfusion mismatch. J Appl Physiol 126(3): 558-568, 2019.

Audi SH, Friedly N, Dash RK, Beyer AM, Clough AV, and Jacobs ER. Detection of hydrogen peroxide production in the isolated rat lung using amplex red. Free Radic Res 52(9): 1052-1062, 2018.

Zhang X, Dash RK^#, Jacobs ER, Camara AKS, Clough AV, and Audi SH^#. Integrated computational model of the bioenergetics of isolated lung mitochondria. PLoS One 13(6):e0197921, 2018. [# Equal contributions for senior authorship].

Korman B, Dash RK, and Peyton PJ. Can mathematical modeling explain the measured magnitude of the second gas effect? Anesthesiology 128(6): 1075-1083, 2018.

Pannala VR, Camara AKS, and Dash RK. Modeling the detailed kinetics of mitochondrial cytochrome c oxidase: Catalytic mechanism and nitric oxide inhibition. J Appl Physiol, 121: 1196-1207, 2016.

Ranji M^#, Salehpour F, Motlagh MM, Sepehr R, Heisner JS, Dash RK, and Camara AKS^#. Optical cryo-imaging reveals a heterogeneous distribution of mitochondrial redox state in the ex vivo guinea pig heart and its alteration during ischemia and reperfusion. IEEE J Trans Eng Health Med 4: 1800210, 2016.

Blomeyer CA, Bazil JN, Stowe DF, Dash RK, and Camara AKS. Mg²⁺ differentially regulates two modes of mitochondrial Ca²⁺ uptake in isolated cardiac mitochondria: Implications for mitochondria Ca²⁺ J Bioenerg Biomemb 48(3):175-178, 2016.

Dash RK, Korman B, and Bassingthwaighte JB. Simple accurate mathematical models of blood HbO₂ and HbCO₂ dissociation curves at varied physiological conditions – Evaluation and comparison with other mathematical models. Eur J Appl Physiol, 116(1): 97-113, 2016.

Salehpour F, Ghanian Z, Yang C, Zhelezenova N, Kurth T, Dash RK, Cowley AW Jr, and Ranji M. Effects of p67phox on the mitochondrial oxidative state in the kidney of Dahl salt-sensitive rats: Optical fluorescence 3D cryoimaging. Am J Physiol Renal Physiol, 309(4): 377-382, 2015

Pannala VR and Dash RK. Mechanistic characterization of the thioredoxin system in the removal of hydrogen peroxide. Free Radic Biol Med 78:42-55, 2015.

Tewari SG, Zhou Y, Otto BJ, Dash RK, Kwok WM, and Beard DA. Markov chain Monte Carlo based analysis of post-translationally modified VDAC gating kinetics. Front Physiol 5:513, 2015.

Bazil JN, Pannala VR, Dash RK, and Beard DA. Determining the origins of superoxide and hydrogen peroxide in the mammalian NADH:ubiquinone oxidoreductase. Free Radic Biol Med 77: 121-129, 2014.

Tewari SG, Camara AKS, Stowe DF, and Dash RK. Computational analysis of Ca²⁺ dynamics in isolated cardiac mitochondria predicts two distinct modes of Ca²⁺ uptake. J Physiol 592(9): 1917-1930, 2014.

Pannala VR, Bazil JN, Camara AKS, and Dash RK. A mechanistic mathematical model for the catalytic action of glutathione peroxidase. Free Radic Res 48(4): 487-502, 2014.

Agarwal B, Dash RK, Stowe DF, Bosnjak ZJ, and Camara AKS. Isoflurane modulates cardiac mitochondrial bioenergetics by selectively attenuating respiratory complexes. Biochim Biophys Acta Bioenerg 1837(3): 354-365, 2014.

Pannala VR, Bazil JN, Camara AKS, and Dash RK. A Biophysically-based mathematical model for the catalytic mechanism of glutathione reductase. Free Radic Biol Med 65: 1385-1397, 2013.

Bazil JN, Blomeyer CA, Pradhan RK, Camara AKS, and Dash RK. Modeling the calcium sequestration system in isolated guinea pig cardiac mitochondria. J Bioenerg Biomembr 45(3): 177-188, 2013.

Agarwal B, Camara AKS, Stowe DF, Bosnjak ZJ, and Dash RK. Enhanced charge-independent mitochondrial free Ca²⁺ and attenuated ADP-induced NADH oxidation by isoflurane: Implications for cardioprotection. Biochim Biophys Acta Bioenergetics 1817(3): 453-465, 2012.

Tewari SG, Dash RK^#, Beard DA, Bazil JN. A biophysical model of the mitochondrial ATP-Mg/Pi carrier. Biophys J 103(7): 1616-1625, 2012. [# Corresponding author]

Pradhan RK, Qi F, Beard DA, and Dash RK. Characterization of Mg²⁺ inhibition of mitochondrial Ca²⁺ uptake by a mechanistic model of mitochondrial Ca²⁺ Biophys J 101(9): 2071-2081, 2011.

Pradhan RK, Beard DA, and Dash RK. A biophysically-based mathematical model for the kinetics of mitochondrial Na⁺-Ca²⁺ Biophys J 98(2): 218-230, 2010.

Dash RK and Beard DA. Analysis of cardiac mitochondrial Na⁺/Ca²⁺ exchanger kinetics with a biophysical model of mitochondrial Ca²⁺ handling suggests a 3:1 stoichiometry. J Physiol 586(13): 3267-3285, 2008.

Detailed Publications and Profiles

Dr. Dash's detailed publications' profiles feature 81 publications, comprised of 76 original articles, three review articles, and two book chapters.

• Frontiers Loop 
• Google Scholar
• MCW Faculty Collaboration Database
• NCBI MyBibliography 
• NCBI PubMed
• Research Gate 

Recent Grants

• Dash, Ranjan K. (Co-Principal Investigator); Cowley, Allen (Co-Principal Investigator). "Experimental and computational analysis of mechanisms of mitochondrial-cellular ROS crosstalk in the kidney in salt-sensitive hypertension."; Sponsored by the National Heart, Lung and Blood Institute (NHBLI) of the National Institutes of Health (January 1, 2021–November 30, 2024).

• Dash, Ranjan K. (Principal Investigator); Terhune, Scott (Co-principal Investigator).  "Predictive Modeling of Cytomegalovirus Replication and Antiviral Efficacy."   Sponsored by NIH/NIAID (March 18, 2020–March 17, 2022).  

• Dash, Ranjan K. (Principal Investigator); Bassingthwaighte, James (Director, Principal Investigator).  "Analyzing the Cardiac Power Grid:  Adaptive Responses to Fluctuating Local Demand."  Sponsored by NIH/NHLBI (September 15, 2015–May 31, 2021).
• Dash, Ranjan K. (Principal Investigator); Bosnjak, Zeljko (Program Director, Principal Investigator). "Project III: Modeling of Mitochondrial and Cellular Function in Anesthetic Cardioprotection", "PPG:  Mechanisms of Anesthetic Cardioprotection."  Sponsored by NIH/NIGMS (March 1, 2015–February 29, 2021).

Past Grants

• Dash, Ranjan K. (Co-Principal Investigator); Przekwas, Andrzej (Principal Investigator). “Systems Biology Based Tools for Modeling Platelet Storage Lesion for Optimal Blood Transfusions.” Sponsored by CFD Research Corporation—US Army (DOD) SBIR Phase II Project (September 26, 2016–January 25, 2019).
• Dash, Ranjan K. (Co-Principal Investigator); Terhune, Scott (Principal Investigator). “Predictive Computational Modeling to Define Antiviral Efficacy.”  Sponsored by Research and Education Program (REP) of Advancing Healthier Wisconsin (AHW) Endowment (February 1, 2017–January 31, 2019).
• Dash, Ranjan K. (Principal Investigator); Camara, Amadou (Co-principal Investigator). “Cations and ROS in Modulating Mitochondrial Function in Normal and Ischemic Hearts.” RO1 Sponsored by the NIH/NHLBI (April 1, 2010–March 31, 2015).
• Dash, Ranjan K. (Co-Principal Investigator); Ozsoyoglu, Meral (Principal Investigator). “Database-Enabled Tools for Regulatory Metabolic Networks.” Sponsored by NSF Biological Database and Informatics (August 1, 2009–July 31, 2012).
• Dash, Ranjan K. (Principal Investigator). “Computational Modeling of Mitochondrial Energetics.” SDG Sponsored by AHA National Affiliate (July 1, 2007–June 30, 2011).